ISSN : 1301-5680
e-ISSN : 2149-8156
TURKISH JOURNAL OF
THORACIC AND
CARDIOVASCULAR SURGERY
Turkish Journal of Thoracic and Cardiovascular Surgery     
Endothelial Seeding as an Method to Improve Patency Rates of Denuded Vascular Surfaces (An Experimental Study)
O. Tansel DARÇIN, Ömer TETİK, Ali RAHMAN, Ahmet ÇEKİRDEKÇİ, *M. Ziya DOYMAZ, **M. Rıza GEZEN
Fırat Üniversitesi Tıp Fakültesi, Göğüs, Kalp ve Damar Cerrahisi Anabilim Dalı
*Temel Mikrobiyoloji Anabilim Dalı
**Histoloji ve Embriyoloji Anabilim Dalı
Endothelial seeding is assumed to be an effective method to improve patency rates of denuded vascular surfaces that is seen after angioplasty. In this study, following anesthesia jugulary veins of eight dogs where harvested bilaterally. From these veins endothelial cells were obtained enzymatically by collagenase and were passaged in to culture mediums until they reach a satisfactory number. After three weeks, dogs were anesthesied similar fashion. Their femoral veins were exposed and experimental intimal denudation were performed bilaterally. Subsequently, one of femoral artery was injected with cell solution and the other with saline solution. Two weeks after injections, arteriographic studies of femoral arteries were performed and arterial specimens were taken for histological evaluation. We concluded that patency rates were statistically significant between study and control sites (p<0.05). Histologic evaluations has also revealed that cultured endothelial cells have attached to the damaged surfaces. There were no cells on the control site. Thrombüs formation was also seen on the damaged subintimal layer despite there was nothing on the study site.
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