ISSN : 1301-5680
e-ISSN : 2149-8156
TURKISH JOURNAL OF
THORACIC AND
CARDIOVASCULAR SURGERY
Turkish Journal of Thoracic and Cardiovascular Surgery     
The antibacterial effect of mesenchymThe antibacterial effect of mesenchymal stem cells on graft infections: An experimental studyal stem cells on graft infections: An experimental study
Mehmet Kabalcı1, İbrahim Deniz Canbeyli2, Erdinç Eroğlu3
1Department of Cardiovascular Surgery, Medicine Faculty of Kırıkkale University, Kırıkkale, Turkey
2Department of Orthopedics and Traumatology, Medicine Faculty of Kırıkkale University, Kırıkkale, Turkey
3Department of Cardiovascular Surgery, Medicine Faculty of Kahramanmaraş Sütçü İmam University, Kahramanmaraş, Turkey
DOI : 10.5606/tgkdc.dergisi.2018.16049
Background: In this study, we aimed to investigate the antibacterial effects of mesenchymal stem cells, compared to tigecycline, on graft infection related with methicillin-resistant Staphylococcus epidermidis in a rat model.

Methods: A total of 42 male adult Wistar rats (age >6 months; weight 300 to 350 g) were divided into six groups including seven rats in each. Group 0 did not undergo any procedure; Group 1 was infected, but untreated; Group 2 was infected and treated with tigecycline without graft placement; Group 3 was infected and received mesenchymal stem cells without graft placement; Group 4 was infected and treated with tigecycline after graft placement; Group 5 was infected and treated with mesenchymal stem cells after graft placement. The pockets created were either left empty or implanted with Dacron grafts. Treatment was commenced at 48 h. Specimens were collected on Day 13. Perigraft tissues were evaluated histopathologically and bacterial colony numbers were counted.

Results: No bacterial colonization was observed in Group 0, whereas there was a significant colonization in Group 1. Complete eradication was achieved in Group 2 and Group 3 (graft-free groups), and near-complete eradication was achieved in Group 4 and Group 5 (graft-implanted groups). The histopathological findings significantly differed between Group 1-Group 2 and between Group 1-Group 3 (graft-free groups). The histopathological findings were similar between Group 2-Group 3 and between Group 4-Group 5.

Conclusion: Our study results suggest that mesenchymal stem cells may be a novel, contemporary alternative to antibiotherapy and may decrease the bio-burden of Staphylococcus at the infected graft areas, and mesenchymal stem cell treatment may be as effective as tigecycline.

Keywords : Infection; mesenchymal stem cell; Staphylococcus epidermidis; tigecycline; wound
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