ISSN : 1301-5680
e-ISSN : 2149-8156
Turkish Journal of Thoracic and Cardiovascular Surgery     
Effects on inflammatory cytokines and oxidative stress markers of pneumoperitoneum performed after thoracotomy in rats
Makbule Ergin, Kürsat Gürlek, Ali Yeginsu
Department of Thoracic Surgery, Medicine Faculty of Gaziosmanpaşa University, Tokat
DOI : 10.5606/tgkdc.dergisi.2012.019
Background: This study aims to investigate the effects of pneumoperitoneum on inflammation after thoracotomy.

Methods: Thirty-two male Wistar type rats weighing 280-320 g were divided into four groups of eight rats each. In the control group rats were only anaesthetized. In thoracotomy group an automatic retractor was inserted for a 30-minute period after left thoracotomy. In the pneumoperitoneum group, 5 ml of room air was injected intraperitoneally. In the thoracotomy + pneumoperitoneum group, thoracotomy was performed and 5 ml of room air was injected intraperitoneally. Blood and lung tissue samples were taken at the 24th hour in all groups and were stored at -80 °C.

Results: In the thorocotomy group, the tumor necrosis factor-alfa, interleukin-6 and protein carbonyl levels were significantly higher than in the control group but no difference was found between the pneumoperitoneum and the thoracotomy + pneumoperitoneum groups.

Conclusion: Pneumoperitoneum performed after thoracotomy does not increase the inflammatory reaction and oxidative stress caused by thoracotomy. Pneumoperitoneum is an effective and easily performed method.

Keywords : Cytokines; oxidative stress markers; pneumoperitoneum; thoracotomy
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