EFFECT OF ACUTE LOWER LIMB ISCHEMIA / REPERFUSION INJURY ON THE  FREE OXYGEN RADICAL  SYSTEMS IN  LUNGS

EFFECT OF ACUTE LOWER LIMB ISCHEMIA / REPERFUSION INJURY ON THE FREE OXYGEN RADICAL SYSTEMS IN LUNGS

İŞBİR Selim, AKGÜN Serdar, AK Koray, CİVELEK Ali, TEKELİ Atike, ÇOBANOĞLU Adnan, *ZEYBEK Ümit, *AYDIN Makbule

Marmara Üniversitesi, Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı,
^*İstanbul Üniversitesi, Deneysel Tıp ve Araştırma Enstitüsü Anabilim Dalı, İSTANBUL

After lower limb ischemia, different pathological changes can be seen in lungs. In this study, we measured the levels of malonyldialdehyde ( MDA ) and Gltathion in lung tissue following the lower limb ischemia / reperfusion injury.

Sixteen Sprague Dawley type rats were divided into two groups. In grup A ( n=8 ) control grup, abdomen was opened and abdominal aorta was explored, and then basal lung MDA and GSH levels were measured. In Grup B (n=8), abdominal aorta was clamped for 45 minutes, then reperfused. In this grup rats were sacrified after 48 hours and their lungs were taken for MDA and GSH analysis. In study group, Statistically significant high MDA levels and in control group statistically low GSH levels means that after acute lower limb ischemia, free oxygen radicals within the lungs were increased and glutathion level, as a protective mechanism, was decreased.

In conclusion, with lower limb I / R injury, free oxygen radical system mediates the pathological changes within lungs beside the other factors.

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