ISSN : 1301-5680
e-ISSN : 2149-8156
TURKISH JOURNAL OF
THORACIC AND
CARDIOVASCULAR SURGERY
Turkish Journal of Thoracic and Cardiovascular Surgery     
Investigating the protective effect of glutamine against cerebral ischemia and bilateral carotid occlusion in rats
Shiraslan Bakhshaliyev1,2, Ergin Arslanoğlu1,3, Görkem Çitoğlu1,2, Mehmet Akif Önalan1,4, Kaan Altunyuva1,4, Çağla Canbay Sarılar1,5, Canan Küçükgergin6, Semra Doğru-Abbasoğlu6, Tuğçe Kıran7, Nilgün Bozbuğa1
1Department of Cardiovascular Surgery, Istanbul University Istanbul Faculty of Medicine, Istanbul, Türkiye
2Department of Pediatric Cardiovascular Surgery, Istanbul Mehmet Akif Ersoy Thoracic and Cardiovascular Surgery Training and Research Hospital, Istanbul, Türkiye
3Department of Pediatric Cardiovascular Surgery, Istanbul Kartal Koşuyolu High Speciality Training and Research Hospital, Istanbul, Türkiye
4Department of Pediatric Cardiovascular Surgery, Dr. Siyami Ersek Thoracic and Cardiovascular Surgery Training and Research Hospital, Istanbul, Türkiye
5Department of Cardiovascular Surgery, Şişli Hamidiye Etfal, Education and Research Hospital, Istanbul, Türkiye
6Department of Biochemistry, Istanbul University Istanbul Faculty of Medicine, Istanbul, Türkiye
7Department of Pathology, Bezmialem Vakıf University Faculty of Medicine Hospital, Istanbul, Türkiye
DOI : 10.5606/tgkdc.dergisi.2022.23089
Background: This study aims to investigate the protective effects of glutamine against cerebral injury resulting from cerebral ischemia-reperfusion by bilateral carotid occlusion in rats.

Methods: Eighteen Wistar albino rats were randomly divided into three equal groups: 1) control group; 2) ischemia-reperfusion group which underwent clamping of the carotid artery for 20 min; and 3) ischemiareperfusion + glutamine group which was treated with two doses of glutamine (1 g/kg) prior to the same clamping procedure as the ischemia-reperfusion group. All rats were sacrificed 24 h after the experiment. Their brain tissue was removed, separated into right and left hemispheres, and sent for analysis. Biochemical analysis was used to determine the oxidant parameters, antioxidant parameters, and glutathione levels in brain tissue. In the histopathological analysis of the brain tissue, ischemic markers such as red neurons, spongiosis, and satellitosis were examined.

Results: Biochemical examination revealed that the levels of malondialdehyde and ferric reducing antioxidant power in the ischemia-reperfusion group were significantly higher than those in the control and ischemia-reperfusion + glutamine groups (p<0.05). The histopathological findings revealed that the levels of red neurons, satellitosis, and spongiosis in the ischemia-reperfusion group were significantly higher than those in the control group (p<0.05). The red neuron and spongiosis levels in the ischemia-reperfusion + glutamine group were significantly higher than those in the control group (p<0.05).

Conclusion: Our study findings indicate that glutamine treatment has a protective effect against ischemia-reperfusion-induced brain damage in rats.

Keywords : Glutamine, ischemia-reperfusion injury, rats
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