ISSN : 1301-5680
e-ISSN : 2149-8156
Turkish Journal of Thoracic and Cardiovascular Surgery     
Oral L-arginine reduces lung injury following reperfusion after ischemia in the lower torso of rats
Gökhan Önem1, Metin Akbulut2, Ahmet Baltalarlı1, Vefa Özcan1, Ercan Gürses3, Şeyda Kaya4, Oya Rendeci1, Mustafa Saçar1
1Departments of Cardiovascular Surgery, Medicine Faculty of Pamukkale University, Denizli
2Departments of Pathology, Medicine Faculty of Pamukkale University, Denizli
3Departments of Anesthesiology, Medicine Faculty of Pamukkale University, Denizli
4Departments of Thoracic Surgery, Medicine Faculty of Pamukkale University, Denizli
Background: Lung injury is known to occur after procedures that involve temporary occlusion of the abdominal aorta and subsequent ischemia/reperfusion (IR). In the present study, we evaluated the effect of oral administration of L-arginine in lung injury caused by lower extremity IR.

Methods: Forty rats were grouped as normal control group (n=10), sham group (n=10), IR injury (IRI) group (n=10) and L-arginine group (n=10). In the IRI and L-arginine groups, infrarenal aorta was cross-clamped for 3 hours followed by 2 hours of reperfusion; rats in L-arginine group received L-arginine monohydrochloride (2.25 mg/kg/day) in drinking water for 7 days prior the experiment. Arterial pH and pCO2 were measured in blood before ischemia, after 90 and 180 minutes of ischemia, and 60 minutes after reperfusion. At the end of the experiment, the right lungs were removed and histologically examined for evidence of lung injury.

Results: There was no significant difference in lung injury score between the normal control and sham groups. IR resulted in a significant increase in lung injury scores in the IRI group and the lungs were less injured in the L-arginine group (3.0±0.6 and 1.5±0.4, respectively, p<0.05). In the blood gas analysis, the IRI group had lower pH than the L-arginine group in reperfusion period (7.28±0.02 and 7.33±0.03 respectively, p<0.05). There was no significant difference in the pCO2 among all four groups.

Conclusion: These data indicate that transient infrarenal aortic occlusion with subsequent ischemia/reperfusion of the lower extremities caused a significant lung injury and that oral administration of L-arginine significantly reduced this injury.

Keywords : Arginine; lung diseases/physiopathology; reperfusion injury/physiopathology
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