Özet

Methods: Between January 2006 and May 2019, a total of 52 patients (44 males, 8 females; mean age: 59.9±9.5 years; range, 42 to 80 years) who underwent lobectomy due to primary lung malignancy were retrospectively analyzed. The N1 lymph nodes were divided into three anatomical groups as hilar, peribronchial, and intraparenchymal. Demographic features, tumor features, follow-up characteristics, and survival and diseasefree survival parameters were analyzed for each group. The results were also examined in terms of number of metastasis, number of metastatic levels, rate of metastasis, and histopathological type.

Results: The five-year survival rate was 66.4% in the peribronchial group and 50% in the hilar group. The five-year disease-free survival rate was 45.7% in the peribronchial group and 37.5% in the hilar group. There was no statistically significant difference between the groups in terms of survival and disease-free survival for anatomical localization, number of metastasis, number of metastatic levels, rate of metastasis, and histopathological type (p>0.05 for all).

Conclusion: The structure that would be formed by examining N1 in terms of parameters such as subtitle levels, number of metastasis, number of metastatic stations, rate of metastasis or combinations of these would have a more impact on the decisions in the follow-up and treatment process in this patient population.

Previous studies regarding the N1 group have mostly addressed survival based on anatomical localization of the tumor and, in some studies, some combinations have been examined, independent of only anatomical localization.[10,14] Evaluation of surgical and oncological results using a high number of parameters would provide more accurate results. Obtaining specific results for subgroups of N1 group would contribute to more precise information about this heterogeneous group. This may be effective in tailoring postoperative management decisions.[15]

In the present study, we aimed to investigate longterm results related to N1 group metastases with respect to anatomical localization and many external parameters such as anatomical localization, number of metastasis (nN), number of metastatic stations (sN), rate of metastasis (lymph node ratio [LNR]), and histopathological type and to examine the effect of these parameters on prognosis.

Yöntem

Initially, N1 lymph nodes were divided into three anatomical groups: hilar, peribronchial, and intraparenchymal. Age, sex, smoking status, disease history, respiratory parameters, size, localization and stage of the tumor, histopathological types, recurrence and metastases, death, chemoradiotherapy status, surgical margin, visceral-vascular invasion, overall survival (OS), and disease-free survival (DFS) data were analyzed for each group. The results were also examined in terms of number of metastasis (nN1a-b) (a:single - b:multiple), number of metastatic levels (sN1a-b) (a:single - b:multiple), rate of metastasis (metastatic lymph node number/total LNR), and histopathological subtype. As the number of intraparenchymal lymph node group was low, the survival and DFS calculations were not calculated, as it could have caused misinterpretation of the statistical results. Due to the low number of recurrence and metastasis, all newly developing lesions secondary to the tumor were used and classified together in the DFS calculation. The classification of the World Health Organization (WHO) was used in histopathological typing and the 8th International Association for the Study of Lung Cancer (IASLC) classification was used in N definitions.

Surgical features
Before surgery, tumors of all patients were diagnosed by transthoracic biopsy or bronchoscopy. Positron emission tomography (PET)-computed tomography (CT), pulmonary function tests (PFTs), echocardiography, and blood tests were performed.

All operations were performed by a posterolateral thoracotomy with mediastinal lymph node dissection. To achieve standardization, only patients who underwent lobectomy were included in the study. A stapler was used while cutting and combining parenchymal adhesions and bronchus. The vessels were tied with silk thread and cut. Tissue adhesives were not used. Lymph nodes were removed by the surgeon during the operation or examined by the pathologist from the specimen in the postoperative period. Postoperatively, the patients were followed by chest radiography and tube thoracostomy. Tube thoracostomies that had no air leakage for more than 24 h and whose daily drainages were less than 100 mL were terminated. In general, if there was no additional condition, the patients were discharged approximately one day after the end of the tube thoracostomy. Survival times were calculated as of the operation day.

Follow-up
After resection, pathology reports were shared with the medical oncology and radiation oncology team and evaluated together. Routinely, a physical examination and chest X-ray follow-up were done at one and three weeks after discharge. Thoracic CT follow-up was performed every three months in the first year, every six months in the second year, and annually thereafter. Abdominal ultrasound and abdominal CT examinations were performed. The PET-CT, magnetic resonance imaging (MRI), bone scintigraphy or biopsy methods were also used for the diagnosis of recurrence and metastasis. The patients who were properly followed by our clinic or whose data were complete and could be found regularly in the central recording system (e-Nabız) were included in the study.

Statistical analysis
Statistical analysis was performed using the IBM SPSS for Windows version 20.0 (IBM Corp., Armonk, NY, USA). The Kolmogorov-Smirnov and Shapiro- Wilk tests were used to assess the assumption of normality. Continuous variables were presented in mean ± standard deviation (SD) or median (25th-75th p ercentile). C ategorical v ariables w ere presented in number and frequency. The relationships between categorical variables were evaluated using the chi-square analysis. Comparisons of continuous variables between the groups were carried out using independent samples t-test or Mann-Whitney U test, where applicable. The Kaplan-Meier method with log-rank test was used for survival analysis. A twosided p value of <0.05 was considered statistically significant.

Bulgular

Table 1: Demographic and clinical features of patients

Histopathological diagnosis of 26 (50%) patients were adenocarcinoma and 26 (50%) were squamous cell carcinoma (SCC). The mean tumor diameter was 2.51±0.98 cm. There was no statistically significant difference among the groups in terms of distribution of the main tumor localization, T stage, histopathological diagnosis, mean tumor diameter, surgical margin, visceral pleural invasion, and vascular invasion (Table 2).

Table 2: Main tumor features

Furthermore, recurrence-metastasis was observed in 18 (34.62%) patients during follow-up (Table 3). There was no statistically significant difference among the groups in terms of recurrence or metastasis, chemotherapy, radiotherapy and mortality rate (Tables 3 and 4). There were 32 (61.54%) peribronchial, 16 (30.77%) hilar, and four (7.69%) intraparenchymal metastatic lymph nodes. The mean number of lymph nodes removed during surgery was 9.13±7.97. There was no statistically significant difference among the groups in terms of mean number of removed lymph node, LNR, nN1a-b, sN1a-b, number of histopathology groups, and recurrencemetastasis rate (Table 4).

Table 3: Features based on follow-up

Table 4: Lymph node features

While local recurrence was observed in 4 (7.69%) patients, systemic spread was observed in 18 (34.62%) patients. There was no statistically significant difference among the groups in terms of total recurrence-metastasis, recurrence-metastasis of nN1a-b groups and histopathological subtypes in terms of recurrence-metastasis in nN1a-b (Table 4).

The mean follow-up was 45.6±32.3 (range, 3 to 140) months. The five-year survival rate was 62.75%, and five-year DFS rate was 42.95% (Figures 1 and 2). There was no statistically significant difference among the groups in terms of survival and DFS for anatomical groups, nN1a-b groups, sN1a-b groups, total histopathological types, and histopathological types on the basis of lymph node groups (Table 5, Figures 1 and 2).

Table 5: Survival and disease-free survival analysis results

Figure 1: Survival analysis. (a) Survival of all patients. (b) Survival for lymph node groups. (c) Survival for the number of metastatic lymph nodes. (d) Survival for the number of metastatic lymph node levels. (e) Relationship between metastatic lymph node ratio and survival. (f) Survival for histopathological types.

Figure 2: Disease-free survival (DFS). (a) DFS of all patients. (b) DFS for lymph node groups. (c) DFS for the number of metastatic lymph nodes. (d) DFS for the number of metastatic lymph node levels. (e) Relationship between metastatic lymph node ratio and DFS. (f) DFS for histopathological types.

In the non-parametric correlation test, there was an inverse proportion among the LNR, and survival and DFS, although it did not reach statistical significance (cc: 0.084, p=0.645; cc: 0.042, p=0.866, respectively) (Table 5, Figures 1 and 2).

Tartışma

Defining the level of dissected lymph node mostly depends on the surgeon's experience and may be a difficult decision. Therefore, in some studies, this situation was attempted to be prevented by classifying lymph nodes as hilar (level 10), interlobar (level 11-12), segmental (level 13-14), or hilar/interlobar-peripherally.[15,17] In our study, N1 lymph nodes were classified as hilar, peribronchial, and intraparenchymal.

In NSCLC, a five-year survival rate of 7 to 67% has been reported in isolated N1 metastasis.[4,7,13,18-20] In the study of Liu et al.,[15] which included 163 patients, 80.4% of whom underwent lobectomy, the three-year and five-year survival rates were 62.1% and 43.5%, respectively. The five-year DFS rate was reported in patients with N1 metastasis at a rate of 50.1 to 52%.[7,17] In our study, the five-year survival rate was 62.75%, and the five-year DFS rate was 42.95%.

In many studies related to the subject, better prognosis is predicted in N1 metastases from hilar to peripheral level.[7,9,15,17,20] In the study of Liu et al.,[15] the five-year survival rate in the hilar/interlobar group was 37.1%, and this rate was 49.9% in peripheral N1 metastases. In the study of Eichorn et al.,[20] the five-year survival rate was 74.2% in hilar and 69.5% in peripheral N1 metastases, and the five-year survival rate was 59.9% in both hilar and peripheral N1 metastasis, and 68.2% in patients with metastasis in the hilar or peripheral group and no statistically significant difference was observed between the groups (p=0.849, p=0.068, respectively).

Lymph node metastases limited to intrapulmonary levels have been reported to show an intermediate prognosis between N0 and N1.[7,16] Similarly, in the study of Rena et al.,[17] the five-year survival rate and DFS rates of intraparenchymal N1 metastasis were found to be located between N0 and N1. Also, in the same study, the five-year survival and DFS rates of level 10 lymph node metastasis were reported to be between the level 11-12 lymph nodes and N2 lymph node metastases. In a study with 120 SCC-diagnosed N1 metastases conducted by Nakao et al.,[4] N1 lymph nodes were classified as direct and discrete groups according to the distance from the main tumor. They reported that the results of the discrete N1 group were almost as poor as N2, although the results of the N1 group were compared with N0 tumors.[4] According to these results, although direct N1 metastasis supports local discrete N1 metastasis to be systemic, both groups were classified as N1.[4] In our study, the five-year survival rate was 52.3% in the hilar group and 67.2% in the peribronchial group. Although the hilar group which was more central in the peribronchial group, a proportional superiority was observed. However, no statistically significant difference was observed between the groups (p=0.302). Similarly, the estimated survival time was longer in the peribronchial group. The fiveyear DFS rates were 38% in the hilar group and 44.8% in the peribronchial group. In the peribronchial group, although the estimated survival time was longer, no significant difference was observed between the groups (p=0.840).

The nN in operable lung cancer has been reported to be an independent prognostic factor.[10,12] There are studies reporting a better outcome of nNa than nNb.[6,7,17] In a study, the five-year survival rate was 67.6% in nNa group and 66.6% in nNb group.[20] In the same study, although there was a better survival tendency in nNa group, there was no significant difference between the groups (p=0.623).[20] Saji et al.[14] reported that a small number (1-3) of N1 metastasis was a better prognostic factor than a higher number of metastases. In this study, the five-year survival and DFS rates were 64.8% and 71.6% in the group with 1-3 number N1 metastases versus 39.2% and 32.9% in the group with four and more N1 metastases (p<0.0001, p=0.0002, respectively).[14] According to some authors, the nN for lung cancers may better express the N category prognosis than the currently used anatomical localization.[3,12,14] In our study, contrary to the literature, the five-year survival and DFS rates of nN1b group (63.33% and 43.04%, respectively) were better than nN1a group (62.23% and 41.56%, respectively). However, there was no statistically significant difference between the groups (p=0.567, p=0.886, respectively). This can be attributed to the high number of nN1b in the peribronchial lymph node group with better prognosis.

The long term outcomes have been reported to be better in patients with sN1a than in cases of sN1b.[7,13,20] In the study of Eichhorn et al.,[20] the five-year survival rate was 58% in sN1a group and 50% in sN1b group. In the study of Maeshima et al.,[7] the five-year DFS rate was 56.3% in sN1a group and 37.8% in sN1b group. Similar to the literature, in our study, the five-year survival and DFS rates were better in sN1a group (62.70% and 42.88%, respectively) than sN1b group (60.58% and 41.78%, respectively). However, there was no statistically significant difference between the groups (p=0.360, p=0.396, respectively).

The LNR is a poor prognostic factor.[7] Indeed, some studies have reported that LNR can be an independent predictor in operable N1 NSCC cases.[6] In the study of Wang et al.,[12] the worst five-year survival rate was found in the group with high LNR, compared to the lower group (p<0.0001). In daily practice, however, it may not be an ideal prognostic factor to use the number of lymph nodes directly or in combination, since there may not be enough N1 lymph node excision or examination in all patients due to different surgical experiences. In contrast, Wang et al.,[12] suggested that the prognostic impact of LNR would less affect the number of dissected lymph nodes. In our study, as the LNR increased, estimated survival and DFS rates decreased, although no statistically significant difference was observed (p=0.645, p=0.866, respectively).

The five-year survival or DFS have been reported to be better in patients with SCC than in cases of adenocarcinoma.[7,9,15,17] In some studies, however, no significant difference was found between the histopathological types in terms of survival time.[15] In a study, central and nN1b had a worse prognosis in the group diagnosed with adenocarcinoma.[20] In this study, the effect of sN1b on survival was observed only in the histopathological type of adenocarcinoma. In our study, the five-year survival rate was 56.48% in SCC group and 69.03% in adenocarcinoma group. The five-year DFS rate was 47.25% in adenocarcinoma group and 38.66% in SCC group. However, there was no statistically significant difference between the two groups (p=0.844, p=0.096, respectively). There was no statistically significant difference in adenocarcinoma and SCC histopathological groups in both peribronchial and hilar LN groups for the five-year survival and DFS rates (p=0.842-0.214, p=0.468-0.332, respectively).

Another issue is that intrapulmonary lymph node metastasis rates have been reported between 12 and 37.5% in the literature, and there is no standard protocol for pathological examination of these metastases.[16] In our study, this rate was 7.69%. Other than routine, repeating gross tissue examination or performing this examination by a second physician (e.g., the surgeon) may increase the rate of pathological lymph nodes. It has been reported that the pathological stage may increase by 2.4% or 11% after a routine or careful pathological examination.[6,16]

According to the reports in the literature, N1 definitions are found to be insufficient in the last lung TNM classification. Evaluations in the form of nN, LNR, and combinations of these among themselves and localization are reported to reflect the prognosis more successfully.[9,14,15,18] Peribronchial station metastasis, nN1a, sN1a, direct invasion, and microscopic invasion are considered good prognostic factors for N1 metastases.[7,17] In our study, although not statistically significant, peribronchial station metastasis, sN1a, low LNR, and histopathological type of adenocarcinoma were good prognostic factors for prognosis.

The present study has some limitations. First, the study is retrospective and our final total number of patients is relatively low. Second, in the study, only patients who underwent resection were included, and there were no results for patients with unoperated N1 metastases. Third, while defining the lymph node localization, the definition of localization of the lymph nodes in the intermediate zones was dependent on the surgeon. In addition, at levels 10, 11, and 12, excision was surgeon-dependent, while 14 and 15 levels were mostly pathologist-dependent.

In conclusion, as in the last Tumor, Node, Metastasis classification, if N1 is considered only in terms of anatomical localization, it may be insufficient to reflect the prognosis accurately. In our study, we found no significant difference in the majority of the criteria analyzed; however, we observed a proportional difference in almost all of the criteria. With the contribution of larger studies in the future, the structure that would be formed by examining N1 in terms of parameters such as sub-levels, number of metastasis, number of metastatic stations, rate of metastasis or combinations of these would have a more impact on the decisions in the follow-up and treatment process of this patient population.

Ethics Committee Approval: The study protocol was approved by the Kocaeli University, Faculty of Medicine, Ethics Committee (Date: 15/06/2020, No: 2020/130). The study was conducted in accordance with the principles of the Declaration of Helsinki.

Patient Consent for Publication: A written informed consent was obtained from each patient.

Data Sharing Statement: The data that support the findings of this study are available from the corresponding author upon reasonable request.

Author Contributions: Idea/concept, design, control, data collection, analysis, literature review - H.F.S., A.E.

Conflict of Interest: The authors declared no conflicts of interest with respect to the authorship and/or publication of this article.

Funding: The authors received no financial support for the research and/or authorship of this article.

Kaynaklar

1) Fukui T, Kato K, Okasaka T, Kawaguchi K, Fukumoto K, Nakamura S, et al. Predictors for hilar/intrapulmonary lymph node metastasis in discrete type of clinical N1 non-small cell lung cancer. Gen Thorac Cardiovasc Surg 2017;65:640-5.

2) Yasukawa M, Kawaguchi T, Kawai N, Tojo T, Taniguchi S. Prognostic significance of mode of nodal involvement in pulmonary pN1 squamous cell carcinoma. Kyobu Geka 2018;71:163-8.

3) Nwogu CE, Groman A, Fahey D, Yendamuri S, Dexter E, Demmy TL, et al. Number of lymph nodes and metastatic lymph node ratio are associated with survival in lung cancer. Ann Thorac Surg 2012;93:1614-9.

4) Nakao M, Yoshida J, Ishii G, Hishida T, Nishimura M, Nagai K. Prognostic impact of node involvement pattern in pulmonary pN1 squamous cell carcinoma patients. J Thorac Oncol 2010;5:504-9.

5) Kang CH, Ra YJ, Kim YT, Jheon SH, Sung SW, Kim JH. The impact of multiple metastatic nodal stations on survival in patients with resectable N1 and N2 nonsmall-cell lung cancer. Ann Thorac Surg 2008;86:1092-7.

6) Zhang J, Liu L, Wang G, Huang C, Chen Y, Zhang Y, et al. New perspective to evaluate N1 staging: The peripheral lymph node metastasis status of non-small cell lung cancer. Thorac Cancer 2019;10:2253-8.

7) Maeshima AM, Tsuta K, Asamura H, Tsuda H. Prognostic implication of metastasis limited to segmental (level 13) and/or subsegmental (level 14) lymph nodes in patients with surgically resected nonsmall cell lung carcinoma and pathologic N1 lymph node status. Cancer 2012;118:4512-8.

8) Riquet M, Pricopi C, Mangiameli G, Arame A, Badia A, Le Pimpec Barthes F. Pathologic N1 disease in lung cancer: The segmental and subsegmental lymph nodes. J Thorac Dis 2017;9:4286-90.

9) Li ZM, Ding ZP, Luo QQ, Wu CX, Liao ML, Zhen Y, et al. Prognostic significance of the extent of lymph node involvement in stage II-N1 non-small cell lung cancer. Chest 2013;144:1253-60.

10) Liu H, Yan T, Zhang T, Chen X, Wang Y, Du J. Proposal of a new nodal classification for operable non-small cell lung cancer based on the number of negative lymph nodes and the anatomical location of metastatic lymph nodes. Medicine (Baltimore) 2019;98:e15645.

11) Asamura H, Chansky K, Crowley J, Goldstraw P, Rusch VW, Vansteenkiste JF, et al. The International association for the study of lung cancer lung cancer staging project: Proposals for the revision of the N descriptors in the forthcoming 8th edition of the TNM classification for lung cancer. J Thorac Oncol 2015;10:1675-84.

12) Wang CL, Li Y, Yue DS, Zhang LM, Zhang ZF, Sun BS. Value of the metastatic lymph node ratio for predicting the prognosis of non-small-cell lung cancer patients. World J Surg 2012;36:455-62.

13) Citak N, Aksoy Y, Isgörücü Ö, Obuz C, Acikmese B, Buyukkale S, et al. A comparison of the currently used nodal stage classification with the number of metastatic lymph nodes and the number of metastatic lymph node stations for non-small cell lung cancer; Which of these is the best prognostic factor? Zentralbl Chir 2020;145:565-73.

14) Saji H, Tsuboi M, Shimada Y, Kato Y, Yoshida K, Nomura M, et al. A proposal for combination of total number and anatomical location of involved lymph nodes for nodal classification in non-small cell lung cancer. Chest 2013;143:1618-25.

15) Liu CY, Hung JJ, Wang BY, Hsu WH, Wu YC. Prognostic factors in resected pathological N1-stage II nonsmall cell lung cancer. Eur Respir J 2013;41:649-55.

16) Wang X, Yan S, Lv C, Wang Y, Wang J, Li S, et al. Impact of omission of intrapulmonary lymph node retrieval on outcome evaluation of lung cancer patients without lymph node metastasis: A propensity score matching analysis. Clin Lung Cancer 2017;18:e411-e416.

17) Rena O, Boldorini R, Papalia E, Turello D, Massera F, Davoli F, et al. Metastasis to subsegmental and segmental lymph nodes in patients resected for non-small cell lung cancer: Prognostic impact. Ann Thorac Surg 2014;97:987-92.

18) Park S, Cho S, Yum SW, Kim K, Jheon S. Comprehensive analysis of metastatic N1 lymph nodes in completely resected non-small-cell lung cancer. Interact Cardiovasc Thorac Surg 2015;21:624-9.

19) Demir A, Turna A, Kocaturk C, Gunluoglu MZ, Aydogmus U, Urer N, et al. Prognostic significance of surgical-pathologic N1 lymph node involvement in non-small cell lung cancer. Ann Thorac Surg 2009;87:1014-22.

20) Eichhorn F, Klotz LV, Muley T, Kobinger S, Winter H, Eichhorn ME. Prognostic relevance of regional lymph-node distribution in patients with N1-positive non-small cell lung cancer: A retrospective single-center analysis. Lung Cancer 2019;138:95-101.