ISSN : 1301-5680
e-ISSN : 2149-8156
Turkish Journal of Thoracic and Cardiovascular Surgery     
Prevention of renal injury due to suprarenal cross-clamping with ischemic preconditioning and carnitine: an experimental study
Demir Çetintaş1, Atilla Aral2, Mustafa Bahadır İnan2, Bilge Ayça Karabörk Kırmızı3, Saba Kiremitçi3, Aslıhan Avcı4, Mustafa Şırlak2, Ahmet Rüçhan Akar2
1Uşak Devlet Hastanesi, Kalp ve Damar Cerrahisi Kliniği, Uşak, Türkiye
2Ankara Üniversitesi Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Ankara, Türkiye
3Ankara Üniversitesi Tıp Fakültesi, Tıbbi Patoloji Anabilim Dalı, Ankara, Türkiye
4Ankara Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Ankara, Türkiye
DOI : 10.5606/tgkdc.dergisi.2016.12251
Background: In this study, we investigated adverse effects of the clamping of suprarenal arteries on the kidney and prevention strategies during abdominal aortic surgery.

Methods: A renal ischemia model was created by clamping the aorta of the suprarenal region of the Wistar rats, as mimicking abdominal aortic surgery. Two cycles of ischemic preconditioning with ischemia for 10 min and reperfusion for 10 min, carnitine and ischemic preconditioning + carnitine protocols were applied. Nephrectomy materials and histopathological and biochemical changes of serum samples were examined.

Results: Renal tubules were the histopathologically and significantly affected regions by ischemia-reperfusion injury. It was found that the ischemic preconditioning procedure consisting of two cycles of ischemia for 10 min and reperfusion for 10 min was suspicious at preventing the reperfusion injury. Oral carnitine was unable to reduce the renal ischemia-reperfusion injury histopathologically; however, it significantly increased the tissue catalase activity.

Conclusion: Ischemic preconditioning and carnitine may prevent renal ischemia-reperfusion injury to a certain extent during abdominal aortic surgery requiring suprarenal cross-clamping.

Keywords : Carnitine; ischemia-reperfusion injury; ischemic preconditioning; suprarenal cross-clamp
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