ISSN : 1301-5680
e-ISSN : 2149-8156
Turkish Journal of Thoracic and Cardiovascular Surgery     
Use of C-type natriuretic peptide as an indicator in detection of inducible peripheral ischemia
Ahmet Çalışkan, Süleyman Yazıcı, Oğuz Karahan, Orkut Güçlü, Orhan Tezcan, Sinan Demirtaş, Bekir Yıldız, Celal Yavuz
Department of Cardiovascular Surgery, Medical Faculty of Dicle University, Diyarbakır, Turkey
DOI : 10.5606/tgkdc.dergisi.2014.9378
Background: In this experimental study, alterations in plasma C-type natriuretic peptide (CNP) levels were evaluated during the critical initial hours of peripheral ischemia.

Methods: Forty male Sprague-Dawley type rats (aged 8 to 12 weeks, weighing 230±30 g) were included in the study. Four groups were created with 10 rats namely control group, group 1, group 2, and group 3. Baseline plasma CNP levels were detected in the control group without any intervention, while the plasma CNP levels were determined in the second hour of peripheral ischemia in group 1. Plasma CNP levels were determined in the fifth hour of peripheral ischemia for group 2, whereas plasma CNP levels were determined in group 3 at the eighth hour of peripheral ischemia.

Results: The baseline plasma CNP levels were 0.285±0.011 pmol/L in the control group. In rats with peripheral ischemia, a significant time-dependent increase was detected in plasma levels of CNP (p<0.05). The plasma CNP levels were detected as 0.350±0.015, 0.486±0.084, and 0.534±0.048 pmol/L in group 1, 2, and 3 respectively.

Conclusion: Plasma CNP, an endothelium-derived vasodilator, is associated with the cellular response in ischemic tissues in a time-dependent manner.

Keywords : C-type natriuretic peptide; ischemic response; peripheral ischemia
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