ISSN : 1301-5680
e-ISSN : 2149-8156
Turkish Journal of Thoracic and Cardiovascular Surgery     
The effect of ozone treatment on remote organ myocardial injury in an aortic ischemia-reperfusion model
Aytuğ Koçyiğit1, Şenol Gülmen2, Tunay Kurtoğlu3, Duygu Kumbul Doğuç4, İbrahim Meteoğlu5, Hüseyin Okutan6, Filiz Alkaya Solmaz71
1Department of Cardiovascular Surgery, Private Denizli Tekten Hospital, Denizli, Turkey
2Department of Cardiovascular Surgery, Medicine Faculty of Süleyman Demirel University, Isparta, Turkey
3Department of Cardiovascular Surgery, Medicine Faculty of Adnan Menderes University, Aydın, Turkey
4Department of Biochemistry, Medicine Faculty of Süleyman Demirel University, Isparta, Turkey
5Department of Pathology, Medicine Faculty of Adnan Menderes University, Aydın, Turkey
6Department of Cardiovascular Surgery, Medikal Park Hospital, Antalya, Turkey
7Department of Anesthesiology and Reanimation, Medicine Faculty of Süleyman Demirel University, Isparta, Turkey
DOI : 10.5606/tgkdc.dergisi.2018.15484
Background: This study aims to investigate the effect of ozone on myocardial ischemia-reperfusion injury occurring after occlusion - reperfusion of infrarenal abdominal aorta in rats.

Methods: Thirty-two Wistar albino rats (weighing 200-250 g) were randomized into four equal groups. The control (sham) group underwent laparotomy and dissection of the infrarenal abdominal aorta without occlusion. Intraperitoneal ozone was applied for 10 days 1 mg/kg/day in the control+ozone group. Afterwards, control+ozone group underwent laparotomy and dissection of the infrarenal abdominal aorta without occlusion. Aortic ischemia-reperfusion and aortic ischemia-reperfusion+ozone groups underwent dissection of the infrarenal abdominal aorta, followed by achieving ischemia and reperfusion by cross-clamping the infrarenal abdominal aorta for 60 minutes and removing the cross-clamp for 60 minutes, respectively. The tissue levels of malondialdehyde and activity levels of superoxide dismutase, catalase, and myeloperoxidase were measured in the myocardial specimens. The tumor necrosis factor, interleukin-6 and troponin-I levels were measured in the plasma. A histopathological examination of the myocardial specimens was undertaken.

Results: Biochemical analysis showed that aortic ischemia-reperfusion significantly increased (p<0.05 vs. control) while ozone significantly decreased (p<0.05 vs. aortic ischemia-reperfusion) the myocardial tissue levels of superoxide dismutase and catalase and level of plasma troponin-I. Histologically, in the aortic ischemia-reperfusion group, myocardial disorganization, myofiber swelling and myofiber eosinophilia in the myocardial tissue samples were significantly increased compared to the control group (p<0.05 vs. control). However, histopathological changes in the aortic ischemia-reperfusion+ozone group decreased compared to the aortic ischemia-reperfusion group.

Conclusion: The results of this experimental study indicate that ozone attenuates myocardial injury and oxidative stress that develop after infrarenal aortic ischemia-reperfusion through three markers; (i) decreased tissue superoxide dismutase and catalase levels, (ii) d ecreased p lasma t roponin-I l evels, a nd (iii) reduced histopathological changes, albeit not statistically significant.

Keywords : Ischemia; myocardial injury; ozone; reperfusion
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