ISSN : 1301-5680
e-ISSN : 2149-8156
Turkish Journal of Thoracic and Cardiovascular Surgery     
Evaluation of gene expression levels in the diagnosis of lung adenocarcinoma and malignant pleural mesothelioma
Gökçen Ömeroğlu Şimşek1, İsmail Ağababaoğlu2, Duygu Dursun1, Selver Özekinci3, Pınar Erçetin1, Hülya Ellidokuz1, Safiye Aktaş1, Duygu Gürel4, İlhan Öztop1, Atila Akkoçlu5
1Department of Basic Oncology, Dokuz Eylül University Faculty of Medicine, İzmir, Turkey
2Department of Thoracic Surgery, Yıldırım Beyazıt University Yenimahalle Training and Research Hospital, Ankara, Turkey
3Department of Pathology, Dicle University Faculty of Medicine, Diyarbakır, Turkey
4Department of Pathology, Dokuz Eylül University Faculty of Medicine, İzmir, Turkey
5Department of Chest Disease, Dokuz Eylül University Faculty of Medicine, İzmir, Turkey
DOI : 10.5606/tgkdc.dergisi.2020.17279
Background: This study aims to evaluate gene expression levels in the diagnosis of lung adenocarcinoma and malignant pleural mesothelioma both which have a distinct treatment and prognosis.

Methods: Between January 2012 and January 2014, 12 newly diagnosed patients with a lung adenocarcinoma, 12 patients with malignant pleural mesothelioma, and eight healthy individuals as the control group were included. After treatment of the fresh samples of lung adenocarcinoma stored at -80°C for ribonucleic acid isolation, and paraffin-embedded tissues of patients with malignant pleural mesothelioma were deparaffinized, complementary deoxyribonucleic acid synthesis and expression of 84 genes associated with deoxyribonucleic acid repair were analyzed via real-time polymerase chain reaction assay. According to the expression of tumor cells, expression of each fold change was calculated.

Results: The BRCA1, BRCA2, CDK7, MLH3, MSH4, NEIL3, SMUG1, UNG, XRCC2, and XRCC4 genes showed more than five-fold higher expression in the patients with lung adenocarcinomas, compared to the control group. The patients with malignant pleural mesothelioma showed a five-fold higher expression in the APEX2, BRCA1, BRCA2, CDK7, MLH1, MLH3, MSH3, MSH4, NEIL3, PARP2, PARP3, PMS1, RAD50, RAD51, RAD51B, RAD51D, RAD52, RPA3, SMUG1, UNG, XPA, XRCC2, and XRCC4 genes, compared to the control group. Comparing malignant pleural mesothelioma with lung adenocarcinoma cases, we found that CDK7, MLH1, TREX1, PRKDC, XPA, PMS1, UNG, and RPA3 genes were overexpressed.

Conclusion: Our study results showed differences between expression profiles of deoxyribonucleic acid repair genes in lung adenocarcinoma and malignant pleural mesothelioma cells. Based on our study results, we suggest that TREX1, PRKDC, and PMS1 genes may play a key role in the differential diagnosis of these two entities.

Keywords : Adenocarcinoma, gene expression, lung, malignant, mesothelioma
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