ISSN : 1301-5680
e-ISSN : 2149-8156
Turkish Journal of Thoracic and Cardiovascular Surgery     
The prognostic significance of histopathologic angioinvasion in stage I non-small cell lung cancer
Çağatay Tezel1, Altuğ Koşar2, Ayşe Ersev2, Yelda Tezel2, Bülent Arman3
1Haydarpaşa Numune Eğitim ve Araştırma Hastanesi, Göğüs Cerrahisi Kliniği, İstanbul
2Süreyyapaşa Göğüs Hastalıkları ve Göğüs Cerrahisi Eğitim ve Araştırma Hastanesi, İstanbul
3Maltepe Üniversitesi Tıp Fakültesi, Göğüs Cerrahisi Anabilim Dalı, İstanbul


Background: We aimed to analyze the prognostic significance of vascular invasion, which is one of the histopathological features affecting the prognosis in early stage lung cancers.

Methods: Forty early stage (T1-2N0M0) non-small cell lung cancer patients (32 males, 8 females; mean age 53.8±8.7 years; range 35 to 69 years) who underwent curative surgery with mediastinal lymph node dissection between December 1997 and January 2004 were retrospectively evaluated. Tumor histology, grade, vascular invasion, and the extent of resections were evaluated. Pathological slides were re-examined and invasion of at least two vascular structures and/or presence of tumoral thrombosis in the lumen of vessels were defined as vascular invasion.

Results: Three-year disease free survival was found to be 67.2±7.5% and five-year survival was 64.5±7.6%. When an evaluation was done considering whether there was vascular invasion or not, there was not a significant difference (Log rank: 0.85; p:0.357; p>0.05) However, adenocarcinoma subtype was found to be related with unfavorable outcome with high local relapses and distant metastases (p<0.01).

Conclusion: Despite the limited number of the cases in our retrospective study, it was found that in early stage lung cancer the type histology was far more prognostic than histological vascular invasion.

Lung cancer is still the most common cause of cancer deaths despite the significant advancements in its treatment. Mortality rates remain high compared to the last 15 years.[1] Numerous studies have been performed on immunohistochemical and histopathologic prognostic factors that may influence the survival in non-small cell lung carcinoma (NSCLC). Vascular invasion determined by histopathologic examination is one of these factors. Detection of factors that influence survival or disease-free survival will elucidate the role of adjuvant therapy in early stage NSCLC, which is a common topic of debate nowadays.[2]


All records of 40 patients (32 males 8 females; mean age 53.8±8.7 years; range 35 to 69 years) with early stage NSCLC (T1-2N0M0) that underwent lung resection with mediastinal lymph node dissection in the Heybeliada Teaching and Research Hospital for Chest Diseases and Thoracic Surgery between December 1997 and January 2004 have been reviewed. Blood tests, radiologic examinations (chest X-ray and thorax computed tomography) and bronchoscopy were performed in all patients. Cranial magnetic resonance imaging and bone scintigraphy was used as preoperative staging modalities when required.

Patients that received neoadjuvant treatment or underwent rethoracotomy for synchronous and metachronous tumors were excluded from the study. Particularly for the tumors of the left-side, patients without mediastinal lymph node metastasis proven by preoperative mediastinoscopy or patients who underwent a preoperative mediastinal lymph node dissection were recruited for the study. Therefore, pathologic stage I was confirmed for all patients with tumors localized on the left side. The follow-up procedure included phone calls to all patients or their relatives to receive up-to-date information. Chest X-rays and thorax computed tomographies were performed to all survivors. No advanced imaging techniques were used in patients that were free of symptoms with normal laboratory results.

All pathological slides were re-examined by one pathologist (AE). Slides of paraffin blocks were stained by standard hematoxylin-eosin. Invasion of at least two vascular structures and/or presence of tumoral thrombosis in the lumen of a vessel in the re-examined slides were defined as vascular invasion.

Statistical analysis of the data was performed by SPSS (Statistical Package for Social Sciences) Windows 10.0 version (SPSS Inc., Chicago, Illionis, USA) software. Data analysis included descriptive statistical methods (mean ± standard deviation) along with the comparison of quantitative data by the Student t-test. The Chisquare and Fisher’s exact Chi-square tests were used for comparison of the qualitative data. The Kaplan Meier survival analysis and the Log rank test were used to evaluate disease-free and overall survival data. Results were presented at a 95% confidence interval and p<0.05 was set as the level of significance.


The demographic characteristics of the patients and the risk factors for vascular invasion observed in 11 patients (27.5%) are presented in Table 1. Excluding the age, no correlation could be established between the gender, T stage, tumor histology, resection size, grade and mortality rates of the patients and the presence of vascular invasion (p>0.05). Vascular invasion showed no correlation with the disease-free survival period either (p>0.05).

Table 1: Characteristics of patients according to vascular invasion

In our study, the three-year and five-year diseasefree survival rates were 67.2±7.5% and 64.5±7.6% respectively. The five-year survival rate of patients with vascular invasion was 54.6±15.0%; six patients (54.6%) in this group survived whereas five died, and the mean survival period was 38 months. The five-year survival rate of patients without vascular invasion was 68.5±8.7%; 20 patients in this group survived (69.0%) whereas nine died, and the mean survival period was 57 months. According to these results, no significant difference could be demonstrated between the overall survival rates of the groups with or without vascular invasion (Log rank: 0.85; p: 0.357; p>0.05), (Fig. 1).

Fig 1: Survival curve analyses.

When each parameter was considered as univariate, the mortality rate was found to be strongly associated with the histologic type (p<0.01; Table 2). The adenocarcinoma group had the highest rate of local recurrence and/or distant metastasis. The relative risk of cases to have adenocarcinoma was calculated as 3.11. Vascular invasion showed no correlation with mortality (p>0.05); and the relative risk for vascular invasion was calculated as 1.46. The histological type was statistically defined as a prognostic factor by the Cox regression analysis (p<0.01; Table 3).

Table 2: Univariate analyses of mortality effecting factors

Table 3: Cox regression analysis


The five-year survival rate of lung cancer that had been 12% between 1974 and 1976 has been slightly improved to a value reaching up to 15% between 1992 and 1997. Nowadays, the lung cancer constitutes 12.8% of all cancers in the world and is responsible for 17.8% of all cancer deaths.[3] Up to now, numerous studies have been performed on histopathologic prognostic factors that may influence survival in non-small cell lung carcinoma.[4-6] Basically, all studies aimed to select patients with early stage tumors that were appropriate for adjuvant treatment following resection.

As reported by the studies on large series, the five-year survival rate of stage I tumors range from 50 to 70%.[7,8] In our study, this figure was found to be 64.5%. Vascular invasions were observed in 11 patients that corresponded to 27.5% of our cases; in other studies, this figure was reported to range from 6.2%[9] to 39%.[10] We were unable to detect any statistically significant effect of vascular invasions on the survival rate in our study. Similarly, Pechet et al.[10] had not been able to demonstrate the effect of vascular invasions on the survival rate by a multivariate analysis in their study involving 100 patients with stage 1 tumor. In another study, Ogawa et al.[11] have shown by both univariate and multivariate analyses that an arterial invasion indicated poor prognosis. However, the details of the study revealed that a wedge resection has been performed on some patients, and no information about vascular invasions in these patients was presented. In our study, we did not discriminate between arterial or venous invasions as in the abovementioned studies. Macchiarini et al.[12,13] have been able to demonstrate the prognostic effect of vascular invasions only for tumors treated by a wedge resection. Subsequent studies from this author revealed that these patients who underwent non-anatomic resections constituted approximately 25% of the cases, which was a considerably high ratio. Conclusions from these studies that showed a negative effect on survival stated that these tumors were more aggressive.

Another reason for investigating the correlation between vascular invasions and the survival rate in early stage tumors is that a vascular invasion can easily be demonstrated by simple histopathologic methods. Many other studies involve non-routine examinations such as assays of complex molecules at the level of nucleotides[14] or immunohistochemical analyses.[15] Considered from this point of view, other factors such as perineural invasions and lymphatic vessel invasions that have the potential to influence the prognosis and can be demonstrated by basic histopathologic methods just like vascular invasions become more important. Sayar et al.[6] have demonstrated the prognostic importance of perineural and lymphatic vessel invasions in the absence of any vascular invasions in their retrospective study on 82 cases. These results are promising and results of larger series are also expected.

Ichinose et al.[16] have assessed vascular invasions separately as arterial, venous and lymphatic vessel invasions and suggested the venous invasion as the predominant prognostic factor. However, they have not described how they discriminated the vascular structures.

Although the differentiation level of a tumor has been shown to have a prognostic effect in various studies,[17] the tumor grade had no affect on the prognosis in our study. Additionally, a study including 2410 patients, 767 of which underwent a complete resection, has revealed a 43.9% five-year survival rate in 417 pN0 patients and the only factor that affected survival was the T stage and N status whereas the histologic type and tumor differentiation were shown to have no effect by multivariate and Cox regression analyses.[18] In the same way, Bakır et al.[19] have failed to demonstrate the prognostic importance of either tumor type or histologic differentiation.

There exist several studies that investigated the effects of various histologic cell types on the prognosis, but their results are inconsistent. In general, studies from the western countries report poor prognosis for squamous cell carcinoma,[20,21] but other studies indicate that histologic type has no effect on the prognosis of tumors of the same stage.[18,19] In our study, we found that adenocarcinoma had poorer prognosis compared to squamous cell carcinoma, as it statistically led to early local recurrence/ distant metastasis at the same early stage.

In conclusion, the retrospective, single-center design of our study with a limited number of patients selected includes some bias that may affect the results. But determining the prevalence of pathologic vascular invasions in early stage NSCLC patients is critical as it shapes the adjuvant treatment at the early stage, which is currently a subject of active debate. Although prognostic importance of vascular invasions could not be demonstrated in the present study, pathologic examination reports should still include the description of any histopathologic vascular invasions in detail. However, studies with larger series are required to find out similar prognostic factors that can be assessed by simple histopathologic examination.


1) Ginsberg RJ, Vokes EE, Raben A. Cancer of the lung. In: DeVita VT Jr, Hellman S, Rosenberg SA, editors. Cancer: principles and practice of oncology. Philadelphia: Lippincott Williams & Wilkins; 1999. p. 849-950.

2) Levine MN, Browman GP, Gent M, Roberts R, Goodyear M. When is a prognostic factor useful? A guide for the perplexed. J Clin Oncol 1991;9:348-56.

3) Jemal A, Thomas A, Murray T, Thun M. Cancer statistics, 2002. CA Cancer J Clin 2002;52:23-47.

4) Hilsenbeck SG, Raub WA Jr, Sridhar KS. Prognostic factors in lung cancer based on multivariate analysis. Am J Clin Oncol 1993;16:301-9.

5) Fujisawa T, Yamaguchi Y, Saitoh Y, Hiroshima K, Ohwada H. Blood and lymphatic vessel invasion as prognostic factors for patients with primary resected nonsmall cell carcinoma of the lung with intrapulmonary metastases. Cancer 1995; 76:2464-70.

6) Sayar A, Turna A, Solak O, Kiliçgün A, Urer N, Gürses A. Nonanatomic prognostic factors in resected nonsmall cell lung carcinoma: the importance of perineural invasion as a new prognostic marker. Ann Thorac Surg 2004;77:421-5.

7) Naruke T, Tsuchiya R, Kondo H, Asamura H. Prognosis and survival after resection for bronchogenic carcinoma based on the 1997 TNM-staging classification: the Japanese experience. Ann Thorac Surg 2001;71:1759-64.

8) van Rens MT, de la Rivière AB, Elbers HR, van Den Bosch JM. Prognostic assessment of 2,361 patients who underwent pulmonary resection for non-small cell lung cancer, stage I, II, and IIIA. Chest 2000;117:374-9.

9) Thomas P, Doddoli C, Thirion X, Ghez O, Payan-Defais MJ, Giudicelli R, et al. Stage I non-small cell lung cancer: a pragmatic approach to prognosis after complete resection. Ann Thorac Surg 2002;73:1065-70.

10) Pechet TT, Carr SR, Collins JE, Cohn HE, Farber JL. Arterial invasion predicts early mortality in stage I non-small cell lung cancer. Ann Thorac Surg 2004;78:1748-53.

11) Ogawa J, Tsurumi T, Yamada S, Koide S, Shohtsu A. Blood vessel invasion and expression of sialyl Lewisx and proliferating cell nuclear antigen in stage I non-small cell lung cancer. Relation to postoperative recurrence. Cancer 1994; 73:1177-83.

12) Macchiarini P, Fontanini G, Hardin JM, Pingitore R, Angeletti CA. Most peripheral, node-negative, non-smallcell lung cancers have low proliferative rates and no intratumoral and peritumoral blood and lymphatic vessel invasion. Rationale for treatment with wedge resection alone. J Thorac Cardiovasc Surg 1992;104:892-9.

13) Macchiarini P, Fontanini G, Hardin MJ, Chuanchieh H, Bigini D, Vignati S, et al. Blood vessel invasion by tumor cells predicts recurrence in completely resected T1 N0 M0 non-smallcell lung cancer. J Thorac Cardiovasc Surg 1993; 106:80-9.

14) Dalquen P, Sauter G, Torhorst J, Schultheiss E, Jordan P, Lehmann S, et al. Nuclear p53 overexpression is an independent prognostic parameter in node-negative non-small cell lung carcinoma. J Pathol 1996;178:53-8.

15) Tezel C, Ersev AA, Kiral H, Urek S, Kosar A, Keles M, et al. The impact of immunohistochemical detection of positive lymph nodes in early stage lung cancer. Thorac Cardiovasc Surg 2006;54:124-8.

16) Ichinose Y, Yano T, Asoh H, Yokoyama H, Yoshino I, Katsuda Y. Prognostic factors obtained by a pathologic examination in completely resected non-small-cell lung cancer. An analysis in each pathologic stage. J Thorac Cardiovasc Surg 1995;110:601-5.

17) Harpole DH Jr, Herndon JE 2nd, Young WG Jr, Wolfe WG, Sabiston DC Jr. Stage I nonsmall cell lung cancer. A multivariate analysis of treatment methods and patterns of recurrence. Cancer 1995;76:787-96.

18) Gonfiotti A, Crocetti E, Lopes Pegna A, Paci E, Janni A. Prognostic variability in completely resected pN1 nonsmall- cell lung cancer. Asian Cardiovasc Thorac Ann 2008; 16:375-80.

19) Bakir K, Uçak R, Tunçözgür B, Elbeyli L. Prognostic factors and c-erbB-2 expression in non-small-cell lung carcinoma (c-erbB-2 in non-small cell lung carcinoma). Thorac Cardiovasc Surg 2002;50:55-8.

20) Nesbitt JC, Putnam JB Jr, Walsh GL, Roth JA, Mountain CF. Survival in early-stage non-small cell lung cancer. Ann Thorac Surg 1995;60:466-72.

21) Postoperative T1 N0 non-small cell lung cancer. Squamous versus nonsquamous recurrences. The Lung Cancer Study Group. J Thorac Cardiovasc Surg 1987;94:349-54.

Keywords : Early stage lung cancer; histopathology; vascular invasion
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