Methods: Eighteen male Wistar albino rats were randomly divided into three groups: the DOBE group (100 mg/kg/ day), the MPFF group (100 mg/kg/day), and the control group . The hearts were perfused twice for 25 minutes before and after 15 minutes of ischemia. Creatine kinase, lactate dehydrogenase, troponin I and myoglobin levels in the coronary perfusates, and malondialdehyde and glutathione levels in the myocardial tissue samples were analyzed. Mean P (mean pressure perfusing coronary arteries), PP (peak systolic pressure), (+)dp/dtmax (contraction strength change over time), (–)dp/dtmax (relaxation strength change over time), time to reach PP, ejection time, and contraction time were calculated.

Results: The mean ‘p' values of the DOBE group were significantly lower than those of the control group at the first and fifth minutes of reperfusion; moreover, PP, (+)dp/ dtmax, and (–)dp/dtmax values were found to be significantly higher than the measurements made at the 15^th, 20^th and 25^th minutes in the control group. In the MPFF group, the (–)dp/dtmax value was significantly higher than that of the control group at the fifth minute of reperfusion.

Conclusion: DOBE is helpful in improving myocardial functions following ischemia and reperfusion.