ISSN : 1301-5680
e-ISSN : 2149-8156
Turkish Journal of Thoracic and Cardiovascular Surgery     
Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion
Gökhan Önem1, Mustafa Saçar1, Hülya Aybek2, Erdoğan Kocamaz3, Fahri Adalı4, Kadir Gökhan Saçkan1, Ahmet Baltalarlı1
1Departments of Cardiovascular Surgery, Medical Faculty of Pamukkale University, Denizli, Turkey
2Departments of Biochemistry, Medical Faculty of Pamukkale University, Denizli, Turkey
3Departments of Histology and Embryology, Medical Faculty of Pamukkale University, Denizli, Turkey
4Department of Cardiovascular Surgery, Balıkesir State Hospital, Balıkesir, Turkey
DOI : 10.5606/tgkdc.dergisi.2012.110
Background: In this study, we aimed to investigate the effects of cilostazol and levosimendan, and the combination of these agents on the lung remote organ damage after ischemia/reperfusion (I/R) following abdominal aortic surgery.

Methods: The experiments were performed on 35 male Wistar albino rats weighing mean 219±26 g. The rats were randomly assigned into five groups, including each of seven rats. Rats were pretreated with cilostazol and levosimendan, alone or in combination, and then lower extremities were subjected to I/R induced by a infrarenal aortic occlusion for duration of 120 minutes, followed by a-60 minute- reperfusion. The rats were sacrificed under deep anesthesia and the lung tissues were removed. Malondialdehyde (MDA) levels, superoxide dismutase (SOD) activity, and glutathione (GSH) levels were measured in the lung tissues. The tissue samples were further examined histopathologically under light microscopy.

Results: It was found that I/R elevated MDA levels accompanied by a reduction in SOD activities and GSH levels (p<0.05). Cilostazol and levosimendan, and their combination restored MDA levels, SOD activity, GSH levels and lung injury scores (p<0.05). There was no significant difference among individual or combined treatment of these agents (p>0.05).

Conclusion: In light of these findings, cilostazol and levosimendan may be useful for protecting the lung tissue from I/R injury, before limb ischemia in vascular surgery. However, the combined use of these agents does not further increase the protection from I/R injury.

Keywords : Abdominal aorta; cilostazol; ischemia/reperfusion; levosimendan; lung injury
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