Endothelial seeding is assumed to be an effective method to improve patency rates of denuded vascular surfaces that is seen after angioplasty. In this study, following anesthesia jugulary veins of eight dogs where harvested bilaterally. From these veins endothelial cells were obtained enzymatically by collagenase and were passaged in to culture mediums until they reach a satisfactory number. After three weeks, dogs were anesthesied similar fashion. Their femoral veins were exposed and experimental intimal denudation were performed bilaterally. Subsequently, one of femoral artery was injected with cell solution and the other with saline solution. Two weeks after injections, arteriographic studies of femoral arteries were performed and arterial specimens were taken for histological evaluation. We concluded that patency rates were statistically significant between study and control sites (p<0.05). Histologic evaluations has also revealed that cultured endothelial cells have attached to the damaged surfaces. There were no cells on the control site. Thrombüs formation was also seen on the damaged subintimal layer despite there was nothing on the study site.