ISSN : 1301-5680
e-ISSN : 2149-8156
Turkish Journal of Thoracic and Cardiovascular Surgery     
Protective effects of melatonin on lung damage associated with one-lung ventilation: An experimental study
Erhan Durceylan1, Ebubekir Aksu1, Hacer Boztepe1, Çiğdem Çengelli2, Evrim Çiftçi Yılmaz3, Emine Dündar Kasapoğlu3, Kevser Erol2, Muammer Cumhur Sivrikoz1
1Department of Thoracic Surgery, Eskişehir Osmangazi University Health Application and Research Hospital, Eskişehir, Turkey
2Department of Medical Pharmacology, Eskişehir Osmangazi University Health Application and Research Hospital, Eskişehir, Turkey
3Department of Medical Pathology, Eskişehir Osmangazi University Health Application and Research Hospital, Eskişehir, Turkey
DOI : 10.5606/tgkdc.dergisi.2020.18261
Background: This study aims to investigate the protective effect of melatonin on lung damage induced by one-lung ventilation in a rat model.

Methods: A total of 20 healthy, Sprague-Dawley male rats were randomized into two equal groups as control (n=10) and melatonin groups (n=10). The control group underwent 60 min of one-lung ventilation, followed by 30 min of two-lung ventilation. In the melatonin group, the rats were administered 10 mg/kg melatonin intraperitoneally 10 min before the start of the experiment. At the end of both ventilation periods, tissue samples were obtained from the lungs of the control and melatonin groups for biochemical analysis and histopathological examinations. Tissue superoxide dismutase, malondialdehyde, and tumor necrosis factor-alpha levels were measured. Lung tissue samples were examined based on the presence and amount of alveolar congestion, intra-alveolar bleeding, and leukocyte and lymphocyte infiltration.

Results: At the end of the study, lung tissue malondialdehyde (3.8±0.9 vs. 1.8±0.8 ?M; p<0.001) and tumor necrosis factor-alpha levels (47.2±15.0 vs. 21.8±7.2 pg/mL; p<0.001) of the melatonin group were found to significantly decrease, compared to the control group. Superoxide dismutase levels of the melatonin group increased at the end of both ventilation periods, and the increase at the end of one-lung ventilation was found to be statistically significant (0.6±0.2 vs. 1.3±0.7 U/mL; p<0.05). Histopathological examination demonstrated that the tissue damage was less in the melatonin group. There was a significant decrease in the alveolar congestion in this group (p=0.0401). Although other histopathological parameters decreased in the melatonin group, no significant difference was found.

Conclusion: Our study results demonstrate that melatonin has protective effects on the lung damage induced by one-lung ventilation both at biochemical and histopathological levels in rats.

Keywords : Histopathology, malondialdehyde, melatonin, one-lung ventilation, superoxide dismutase, tumor necrosis factor-alpha
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