ISSN : 1301-5680
e-ISSN : 2149-8156
Turkish Journal of Thoracic and Cardiovascular Surgery     
The effect of rutin on pulmonary contusion induced by blunt trauma in rats: Biochemical and histopathological evaluation
Bekir Elma1, Renad Mammadov2, Yasin Bilgin3, Gülce Naz Yazıcı4, Halis Süleyman2, Taha Abdulkadir Çoban5
1Department of Thoracic Surgery, Medicine Faculty of Erzincan Binali Yıldırım University, Erzincan, Türkiye
2Department of Pharmacology, Medicine Faculty of Erzincan Binali Yıldırım University, Erzincan, Türkiye
3Emergency Health Services, Erzincan Health Directorate, Erzincan, Türkiye
4Department of Histology and Embryology, Medicine Faculty of Erzincan Binali Yıldırım University, Erzincan, Türkiye
5Department of Biochemistry, Medicine Faculty of Erzincan Binali Yıldırım University, Erzincan, Türkiye
DOI : 10.5606/tgkdc.dergisi.2022.21992
Background: This study aims to investigate the possible protective effects of rutin, also called vitamin P1, against pulmonary contusion induced by blunt chest trauma in a rat model.

Methods: Thirty male albino Wistar rats were separated into three equal groups as healthy group, trauma group, and trauma+rutin group. After anesthesia provided by intraperitoneal administration of 60 mg/kg ketamine and xylazine by inhalation at appropriate intervals, 200 g weight was dropped from 1 m height to the anterior chest wall of the animals in the trauma group (n=10) and trauma+rutin group (n=10) and pulmonary contusion was created. Thirty min after the trauma, 50 mg/kg of rutin was administered into the stomach of trauma+rutin group animals orally with gavage. The rats received rutin once daily for two days and were sacrificed 48 h later. Their lung tissues were removed and examined biochemically and histopathologically.

Results: Nuclear factor-kappa B, cyclooxygenase-2, and malondialdehyde levels increased in the trauma group compared to the healthy group, and rutin administration prevented this increase. Total glutathione levels decreased in the trauma group, and rutin administration also prevented this decrease. The histopathological findings were compatible with the biochemical findings.

Conclusion: Our study results suggest that rutin has a protective effect on contused lung tissue in rats.

Keywords : Blunt thoracic trauma, pulmonary contusion, rat, rutin
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