ISSN : 1301-5680
e-ISSN : 2149-8156
Turkish Journal of Thoracic and Cardiovascular Surgery     
Vitamin C and Iloprost Attenuate Skeletal Muscle Injury Caused By Ischemia-reperfusion of The Lower Extremities
Mustafa Saçar 1, Vefa Özcan 1, Hülya Aybek 2, Gökhan Önem1, Süleyman Demir 2, İbrahim Gökşin 1, Derviş Verdi 1, Ahmet Baltalarlı1
1Departments of Cardiovascular Surgery Medicine Faculty of Pamukkale University, Denizli
2 Departments of Biochemistry, Medicine Faculty of Pamukkale University, Denizli
Background: We evaluated the effect of iloprost and vitamin C on the reperfusion injury to the skeletal muscle, using an ischemic revascularized hindlimb model in rats.

Methods: Thirty-four Wistar albino rats were divided into five groups. Three groups were assigned to ischemic/reperfusion (I/R) injury via cross-clamping of the abdominal aorta just below the renal arteries for three hours, followed by one hour of reperfusion. Of these, one group (n=8) of rats received 100 mg/kg ascorbic acid via the left jugular vein before cross-clamping, while another group (n=8) received 20/ng/kg/min iloprost by constant intravenous infusion via the left jugular venous cannula during cross-clamping. In the sham group (n=6), the rats were anesthetized, a left jugular venous cannula was inserted and the abdomen was left open during the same period. In the control group (n=6), the soleus muscles were removed and blood samples were taken immediately after sternotomy without any further treatment.

Results: Compared to the I/R group, arterial blood pO2 and HCO3 levels were significantly high (p<0.05) and skeletal muscle malondialdehyde (MDA), plasma MDA, lactate dehydrogenase (LDH), and creatine phosphokinase (CPK) levels were significantly low (p<0.05) in both the iloprost and vitamin C groups. Vitamin C-treated rats had significantly lower skeletal muscle MDA levels than those of the iloprost group (p<0.05). No significant differences were found between the iloprost and vitamin C groups with regard to pO2, HCO3, plasma MDA, LDH and CPK (p>0.05).

Conclusion: Ischemia and reperfusion of the lower extremity result in significant skeletal muscle injury. This injury can be attenuated by both vitamin C and iloprost pretreatment.

Keywords : Ascorbic acid/therapeutic use; disease models, animal; iloprost/therapeutic use; ischemia; muscle, skeletal; peripheral vascular diseases; rats; reperfusion injury
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