ISSN : 1301-5680
e-ISSN : 2149-8156
Turkish Journal of Thoracic and Cardiovascular Surgery     
Ven çıkarma tekniklerinin safen ven greftlerinde endotel inflamasyonu ve nitrik oksit sentezi üzerine etkileri
Sadi Kaplan1, Gianluigi Bisleri1, Kamer Kılınç2, Adnan Çobanoğlu3, Mehmet C. Öz1
1Division of Cardiothoracic Surgery, College of Physicians & Surgeons, Columbia University, New York, NY, USA
2Department of Biochemistry, Medical Faculty of Hacettepe University, Ankara, Turkey
3Department of Cardiovascular Surgery, Türkiye Yüksek İhtisas Training and Research Hospital, Ankara, Turkey
DOI : 10.5606/tgkdc.dergisi.2013.7056


Background: In this study, we investigated the effects of harvesting technique in saphenous vein grafts (SVG) (either conventional or endoscopic) on endothelial integrity, inflammation, and nitric oxide (NO) synthesis.

Methods: Segments of 20 SVGs were harvested from 20 patients (23 males, 17 females; mean age 65.6±6.3 years; range 43 to 78 years) undergoing coronary artery bypass graft (CABG) surgery with the endoscopic (group 1) or conventional (group 2) technique. Saphenous vein specimens were stored in heparinized blood for one hour at the room temperature. As a marker of preserved endothelial function, nitric oxide (nitrate, NO3 and nitrite, NO2) levels in the SVGs were measured by means of the Greiss method. Saphenous tissual myeloperoxidase (MPO) activity, as a marker of neutrophil infiltration into the saphenous vein graft endothelium, was also measured in each group. This measurement revealed the extent of SVG endothelial damage and inflammation resulting from neutrophils.

Results: Nitric oxide formation, as NO3 plus NO2, (group 1=34.18±5.43 mM versus group 2=25.73±2.52 mM, p<0.001) was higher in endoscopically harvested SVGs as compared to conventionally harvested SVGs. Saphenous tissual MPO activity (group 1=6.71±0.86 nm/min versus group 2=9.11±0.94 nm/min; p<0.001) was significantly lower in endoscopically harvested SVGs as compared with conventionally harvested grafts.

Conclusion: Neutrophil infiltration into the vascular endothelium and neutrophil-induced endothelial injury is reduced in endoscopically harvested SVGs. Also, endothelial NO synthesis is better preserved in endoscopically harvested SVGs. These results suggest that endoscopic harvesting techniques can be used without major detrimental effects on vascular endothelial function and integrity in SVGs.

Endoscopic saphenous vein harvesting during coronary artery bypass grafting (CABG) surgery has been steadily gaining popularity over the last several years. Previous studies have demonstrated that this type of approach reduces the incidence of wound complications associated with conventional harvesting while providing superior aesthetic results[1-5] combined w ith acceptable and comparable early and long-term graft patency rates.[6,7] Additionally, histopathological studies have failed to depict considerable differences between endoscopically and conventionally harvested vein grafts.[1,8-11] Although the endothelial layer appears grossly intact after minimally invasive harvesting techniques,[8-11] the evaluation of the endothelial function of saphenous veins with more reliable markers has been limited. Neutrophilendothelial cell interaction plays an important role in endothelial injury and the pathogenesis of saphenous vein graft (SVG) occlusions.[12,13] It has been demonstrated that among other factors, decreased endothelial nitric oxide (NO) synthesis due to endothelial injury[14] can significantly influence adhesion molecule expression and subsequent neutrophil-induced endothelial injury. In fact, leukocyte adhesion to the endothelium is regulated by the presence of leukocyte-endothelial cell adhesion molecules on the surface of endothelial cells, such as intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), P-selectin, and circulating neutrophils (CD11b/CD18).[13,15] Under in vivo physiological conditions, none of the adhesion molecules, or only a relative few, are expressed on the intact SVG endothelium,[13] although enhanced adhesion molecule expression has been observed in SVGs undergoing late occlusion.[16]

Nitric oxide, generated from L-arginine in the endothelial cells, is a reliable marker of endothelial function[17,18] since it activates guanylyl cyclase and increases intracellular concentrations of cyclic guanine monophosphate (cGMP) in platelets and smooth muscle cells.[17,18] In addition, NO plays a significant role in leukocyte-endothelium interactions[14,17,18] and in vascular tone regulation.[18] Moreover, it is believed that endogenous NO and cGMP might contribute in the reduction of inflammation in vivo through the suppression of endothelial adhesion molecule expression. Previous studies have demonstrated that surgical harvesting of saphenous veins[15,18-20] as well as storagerelated hypoxia-ischemia[15,16,21] impairs or completely abolishes the endothelial NO synthesis in SVGs.

The present study was therefore designed to evaluate the effects of both the endoscopic and conventional harvesting techniques on SVG endothelial function and integrity by measuring the endothelial NO synthesis and the activity of myeloperoxidase (MPO), an enzyme occurring in neutrophils, monocytes, and macrophages.


Study population
Forty patients undergoing elective, non-emergency, on-pump CABG surgery were prospectively randomized into either the endoscopic vein harvest group (group 1, n=20; mean age 66.2±5.7 years) or the open vein harvest group (group 2, n=20; mean age 64.6±6.9 years). The criteria for exclusion from the study were diabetes mellitus (DM), peripheral arterial obstructive disease (PAOD), varicose veins, or the recent use of steroids. This study was conducted in accordance with approval from the Columbia University Institutional Review Board (IRB 0988) and in conformance with the Declaration of Helsinki. The procedures were also performend with the patients’ informed consent.

Surgical techniques
In group 1, all patients’ legs were circumferentially prepared with a povidone- iodine solution, and their feet were placed in sterile stockinettes. Before surgery, 5000 units of intravenous heparin were administered to each patient. The endoscopic vein harvesting was performed with the VASOVIEW Endoscopic Vessel Harvesting System (Guidant Corporation, Santa Clara, California, USA), which uses carbon dioxide (CO2) insufflation for visualization and dissection. To begin the procedure in group 1, a 1.5 to 2 cm incision was made medially above or below the knee, depending on the length of the vein required. Harvesting was directed toward the groin region. The side branches were divided by using bipolar cauterizing scissors or a bisector. A small puncture was then made under endoscopic guidance proximally over the saphenous vein, which was then clamped and divided, and the proximal end was ligated. After removing the vein from the leg, the side branches were ligated with 4/0 silk ties. Finally, the incisions were closed with absorbable subcutaneous and subcuticular sutures and wrapped with an elastic Ace bandage for 24 hours.

In group 2, a longitudinal incision was made over the course of the saphenous vein starting at the groin region. Once exposed, the side branches were ligated with 4/0 silk ties and then divided. The wound was closed in layers by using absorbable sutures and also wrapped with an Ace bandage for 24 hours. All veins were gently distended manually with autologous heparinized blood, and any avulsed branches were repaired by carefully approximating the adventitial layer with 7/0 polypropylene sutures. The veins were then placed in a heparinized blood solution until they were needed. Both the open and the endoscopic harvesting of the venous conduit were performed by a surgeon with consistent experience with these techniques.

In both groups, a 2 cm sample was taken from the groin end of the vein immediately after its removal from the leg and before any further manipulation, such as distention after harvesting. Tubes containing 5 mL of heparinized autologous blood were prepared, and the samples were then stored at room temperature for 60 minutes, which is comparable with the average storage period for a harvested vein before implantation as a bypass conduit. Each specimen was rinsed briskly in normal saline solution three times, divided into two approximately equal parts (one segment for nitrite and nitrate measurements and one for MPO activity determination), snap-frozen in liquid nitrogen, and stored separately in a –80 °C freezer until analysis.

Nitrite/nitrate assay
The tissue samples were diluted with equal volume of phosphate buffered saline (PBS) and filtered by centrifugation using Amicon 30 kDa cut-off filters (Millipore Corporation. Bedford, Massachusetts, USA). For the determination of nitrite plus nitrate concentration in the filtrates, the Greiss method using the nitrate reductase catalyzed conversion of nitrate to nitrite was adopted.[22] After the conversion of nitrate into nitrite, the Greiss reagent was added to the samples, and the absorbance was measured at 540 nm. Then the nitrite and nitrate standards were studied to calculate tissue nitrite and nitrate concentration.

Myeloperoxidase assay
To determine the neutrophil adhesion to SVG endothelium after harvesting and the one-hour storage period, SVG tissue MPO activity was measured using the method described previously by Mullane et al.[23] The MPO enzymatic activity was measured spectrophotometrically at 460 nm using a PowerWaveX microplate reader (Biotek Instruments, Winooski, Vermont, USA), and the result was expressed as ΔAbs 460 nm/min. The MPO value was expressed as the mean ± SD of duplicate determinations, and all assays were measured without prior knowledge as to the group origin of each of the vein samples.

Statistical analysis
All statistics were obtained using the Statistical Package for the Social Sciences (SPSS Inc, Chicago, Illinois, USA) version 10.0 software program. All continuous variables were expressed as mean ± standard deviation. Comparisons between groups were made by either Student’s t-test or the Mann-Whitney U test.


In this study, endoscopic saphenous vein procurement was performed without complications in all patients. The time to harvest the saphenous vein (harvesting time plus skin closure time) was significantly shorter in group 1 (38.46 minutes) versus group 2 (50.47 minutes) (p<0.001).

Nitric oxide formation, nitrate plus nitrite release
Greiss assays were performed to quantify the NO level of the saphenous veins, and the total NO (nitrate plus nitrite) levels were higher in group 1 (34.18±5.43 μM than in group 2 (25.73±2.52 mM) (p<0.001) (Figure 1).

Figure 1: Human saphenous vein graft total nitric oxide (nitrate plus nitrite) synthesis after harvesting and the one-hour waiting period. To evaluate the effects of the harvesting technique (either endoscopic or conventional) on SVGs, endothelial function and nitric oxide (nitrate, NO3 a nd n itrite, N O2) levels in the vein grafts were measured. The data for the samples is shown as group mean ± SD. For each group, n=20.

Myeloperoxidase activity
The mean MPO activity was significantly lower in tissue segments from group 1 (6.71±0.86 nm/min) compared with those in group 2 (9.11±0.94 nm/min; p<0.001) (Figure 2).

Figure 2: Human saphenous vein graft neutrophil accumulation after harvesting and the one-hour waiting period. Myeloprexidase activity (change in absorbance-ΔAbs at 460 nm/min) was determined to quantify neutrophil deposition in the vein grafts. The data for the samples is shown as group mean ± SD. For each group, n=20.


The saphenous vein is still extensively used as a bypass conduit in CABG surgery even though the long-term patency rate is inferior to that of internal mammary artery (IMA) grafts. The poor long-term patency of SVGs, a primary reason for the current trend toward the use of arterial grafts, has been attributed to the structural and functional characteristics of the vascular wall and the endothelium of saphenous veins.[24,25] Therefore, the importance of an atraumatic preparation of the saphenous vein has been recognized for many years. In general, saphenous vein harvesting and the preparation techniques associated with it have been considered as potential sources of trauma that may accelerate the process of graft failure after implantation as bypass conduits.[24,25] Surgical preparation of SVGs for bypass grafting involves exposure, dissection, intraluminal distension, side branch ligation, and storage in a physiological solution; however, this invariably causes a high degree of damage to the endothelium. In particular, excessive surgical manipulation of saphenous veins during surgical preparation injures the endothelial and medial layers,[25,26] reduces NO bioavailability,[25] and results in platelet and leukocyte adhesion and smooth muscle cell migration into the intima.[26] These changes contribute to the accelerated atherosclerotic disease leading to graft failure.[27]

Additionally, the morbidity associated with conventional vein harvesting is high because the open technique requires long incisions and leads to significant wound morbidity, with an incidence rate varying from 3-44%. Additional problems with this technique include hematoma, dehiscence, cellulitis, skin necrosis, neuralgia, and infection.[5]

Several studies have demonstrated that the endoscopic approach avoids traction on the vein while being harvested, significantly minimizes the length of incisions and blood loss, and reduces the incidence of leg wound infection and complications when compared with the traditional open vein harvesting technique.[1-8,28] Moreover, endoscopic harvesting provides major benefits to patients through means of pain reduction and superior aesthetic results[1-8,28] while also having satisfactory early and long-term patency rates.[6,7]

Preservation of saphenous vein functionality by means of minimally invasive harvesting might have important implications for the immediate functional integrity and long-term patency rate of venous grafts. Leukocyte-endothelial cell interaction is one of the primary determinants of endothelial injury. Adhesion molecules, expressed on endothelial cells, mediate the binding of leukocytes to endothelial cells through interactions with their counterreceptors on the leukocytes.[13] As previously state, under in vivo physiological conditions, adhesion molecule expression on segments of recently harvested saphenous veins (endoscopic or conventional) is very low or almost absent.[13,29,30] However, during the storage period, SVG endothelial cells retain their ability to synthesize leukocyte chemoattractants such as interleukin-8[1] while the endothelial disruption upregulates the expression of such molecules.[12,21] Therefore, preservation of endothelial integrity during harvesting and storage along with the attenuation of adhesion molecule expression before implantation may be potentially beneficial in preventing leukocyte adhesion to the vessel wall and suppressing neutrophil-mediated endothelial injuries in SVGs after implantation as bypass conduits. In a previous study, we demonstrated that after one-hour storage of endoscopically harvested vein grafts, it is possible to determine visible endothelial cell adhesion molecules, ICAM-1, and VCAM-1 as well as inducible NO synthase-2 (INOS-2) expression.[29,30] Therefore, in this study, we again stored the vein samples for a onehour period before measuring leukocyte infiltration in the vascular endothelium of the SVGs. By comparing matched pairs of vein tissue, we demonstrated that harvesting and a 60-minute period of storage in heparinized blood results in increased leukocyte infiltration into the endothelium in SVGs harvested by means of both endoscopic and conventional techniques, indicating an increase in the proinflammatory reaction in these vein grafts in the endothelium. In contrast, when compared with conventional harvesting, endoscopic harvesting seemed to provide better endothelial cell function, as depicted by decreased myeloperoxidase activity, indicating less neutrophil infiltration.

Additionally, our study aimed to evaluate the difference in NO production following conventional or endoscopic saphenous vein harvesting. Endothelial nitric oxide synthase (NOs) transcription is increased by hypoxia.[17] Inducible NOs is found in various tissues, such as vein grafts, and its expression is induced by immunoactivation of neutrophils, macrophages, and monocytes.[17] Their induction generally reflects a pathophysiological cellular response to immunoactivation and elicits vasoplegia, myocardial depression, and cytotoxic effects.[17] The main property of NO is to inhibit the expression of adhesion molecules (such as selectins, GPIIbIIIa, ICAM, and VCAM) in platelets, neutrophils, and vascular tissues.[18] Harvesting and hypoxic storage reduce endothelial NOs expression and decrease NO production in SVGs,[14,16,19,20] causing an upregulation of leukocyte adhesion molecules on SVG endothelial cells.[12,16] It has been previously demonstrated that surgical damage to the endothelium results in a loss of endothelial-derived NO, which increases neutrophil and monocyte adhesion to the vessel wall and vasospasm.[25] Liu et al.[20] investigated the effect of surgical preparation of the conventionally harvested saphenous vein on NO release from the endothelium by direct measurement of NO. They demonstrated that mechanical distention during surgical preparation almost abolishes NO release (both the basal and stimulated NO), thus holding potential implications for the long-term patency rate of the vein graft.[20]

Therefore, to evaluate the effect of endoscopic and conventional harvesting on saphenous vein endothelium, we measured the total NO levels in the endoscopically and conventionally harvested grafts since this serves as a much more reliable marker of endothelial integrity and function. We demonstrated that the levels were significantly higher in endoscopically harvested SVGs compared with the conventionally harvested grafts, which implies a more preserved endothelial function in the endoscopically harvested conduits.

Studies evaluating endothelial function after minimally invasive vein harvesting have been limited; therefore, the impact of endoscopic harvesting on endothelial function is unclear. Griffith et al.[9] showed similar endothelial, elastic lamina, and smooth muscle continuity as well as medial and adventitial connective tissue uniformity between conventionally harvested versus endoscopically harvested veins. In a large group of patients, Crouch et al.[31] reported that there was no consistent decrease in vein integrity after performing the endoscopic harvesting technique. Moreover, Yun et al.[7] and Davis et al.[6] determined the long-term patency rates of endoscopically harvested SVGs and reported that this technique provided a comparable or even higher patency rates in comparison with traditionally harvested veins. These studies clearly prove that the endoscopic harvesting technique does not induce more significant histological trauma than that observed during traditional saphenectomies.

In conclusion, our study demonstrates that endoscopic harvesting reduces neutrophil infiltration and neutrophilinduced endothelial injury into the vascular endothelium of SVGs. Furthermore, our study shows that endothelial NO formation is better preserved with the endoscopic harvesting technique than with conventional harvesting. Finally, better preservation of endothelial NO synthesis and reduction of leukocyte-induced endothelial inflammation by endoscopic harvesting suggests that minimally invasive harvesting improves the quality of saphenous veins and leads to potential benefits in terms of the long-term patency of SVGs.

The authors wish to thank Erdem Karabulut, PhD, Department of Biostatistics, Hacettepe University, Ankara, Turkey, for the statistical analysis.

Declaration of conflicting interests
The authors declared no conflicts of interest with respect to the authorship and/or publication of this article.

This study was supported by Institutional Research Funds of Columbia University. Doctor Kaplan was supported by a research grant from the Turkish Ministry of Health.


1) Kiaii B, Moon BC, Massel D, Langlois Y, Austin TW, Willoughby A, et al. A prospective randomized trial of endoscopic versus conventional harvesting of the saphenous vein in coronary artery bypass surgery. J Thorac Cardiovasc Surg 2002 ;123:204-12.

2) Carpino PA, Khabbaz KR, Bojar RM, Rastegar H, Warner KG, Murphy RE, et al. Clinical benefits of endoscopic vein harvesting in patients with risk factors for saphenectomy wound infections undergoing coronary artery bypass grafting. J Thorac Cardiovasc Surg 2000;119:69-75.

3) Davis Z, Jacobs HK, Zhang M, Thomas C, Castellanos Y. Endoscopic vein harvest for coronary artery bypass grafting: technique and outcomes. J Thorac Cardiovasc Surg 1998;116:228-35.

4) Bitondo JM, Daggett WM, Torchiana DF, Akins CW, Hilgenberg AD, Vlahakes GJ, et al. Endoscopic versus open saphenous vein harvest: a comparison of postoperative wound complications. Ann Thorac Surg 2002;73:523-8.

5) Athanasiou T, Aziz O, Skapinakis P, Perunovic B, Hart J, Crossman MC, et al. Leg wound infection after coronary artery bypass grafting: a meta-analysis comparing minimally invasive versus conventional vein harvesting. Ann Thorac Surg 2003 ;76:2141-6.

6) Davis Z, Garber D, Clark S, Roth H, Bufalino V, Budoff MJ, et al. Long-term patency of coronary grafts with endoscopically harvested saphenous veins determined by contrast-enhanced electron beam computed tomography. J Thorac Cardiovasc Surg 2004;127:823-8.

7) Yun KL, Wu Y, Aharonian V, Mansukhani P, Pfeffer TA, Sintek CF, et al. Randomized trial of endoscopic versus open vein harvest for coronary artery bypass grafting: six-month patency rates. J Thorac Cardiovasc Surg 2005;129:496-503.

8) Cable DG, Dearani JA, Pfeifer EA, Daly RC, Schaff HV. Minimally invasive saphenous vein harvesting: endothelial integrity and early clinical results. Ann Thorac Surg 1998;66:139-43.

9) Griffith GL, Allen KB, Waller BF, Heimansohn DA, Robison RJ, Schier JJ, et al. Endoscopic and traditional saphenous vein harvest: a histologic comparison. Ann Thorac Surg 2000;69:520-3.

10) Black EA, Guzik TJ, West NE, Campbell K, Pillai R, Ratnatunga C, et al. Minimally invasive saphenous vein harvesting: effects on endothelial and smooth muscle function. Ann Thorac Surg 2001;71:1503-7.

11) Meyer DM, Rogers TE, Jessen ME, Estrera AS, Chin AK. Histologic evidence of the safety of endoscopic saphenous vein graft preparation. Ann Thorac Surg 2000;70:487-91.

12) Verrier ED, Boyle EM Jr. Endothelial cell injury in cardiovascular surgery. Ann Thorac Surg 1996;62:915-22.

13) Krieglstein CF, Granger DN. Adhesion molecules and their role in vascular disease. Am J Hypertens 2001;14:44S-54S.

14) Suematsu M, Tamatani T, Delano FA, Miyasaka M, Forrest M, Suzuki H, et al. Microvascular oxidative stress preceding leukocyte activation elicited by in vivo nitric oxide suppression. Am J Physiol 1994;266:H2410-5.

15) Motwani JG, Topol EJ. Aortocoronary saphenous vein graft disease: pathogenesis, predisposition, and prevention. Circulation 1998;97:916-31.

16) Thatte HS, Khuri SF. The coronary artery bypass conduit: I. Intraoperative endothelial injury and its implication on graft patency. Ann Thorac Surg 2001;72:S2245-52.

17) Vural KM, Bayazit M. Nitric oxide: implications for vascular and endovascular surgery. Eur J Vasc Endovasc Surg 2001;22:285-93.

18) Napoli C, Ignarro LJ. Nitric oxide and atherosclerosis. Nitric Oxide 2001;5:88-97.

19) Tsui JC, Souza DS, Filbey D, Bomfim V, Dashwood MR. Preserved endothelial integrity and nitric oxide synthase in saphenous vein grafts harvested by a ‘no-touch’ technique. Br J Surg 2001;88:1209-15.

20) Liu ZG, Liu XC, Yim AP, He GW. Direct measurement of nitric oxide release from saphenous vein: abolishment by surgical preparation. Ann Thorac Surg 2001;71:133-7.

21) Schaeffer U, Tanner B, Strohschneider T, Stadtmüller A, Hannekum A. Damage to arterial and venous endothelial cells in bypass grafts induced by several solutions used in bypass surgery. Thorac Cardiovasc Surg 1997;45:168-71.

22) Grisham MB, Johnson GG, Lancaster JR Jr. Quantitation of nitrate and nitrite in extracellular fluids. Methods Enzymol 1996;268:237-46.

23) Mullane KM, Kraemer R, Smith B. Myeloperoxidase activity as a quantitative assessment of neutrophil infiltration into ischemic myocardium. J Pharmacol Methods 1985;14:157-67.

24) He GW. Arterial grafts for coronary artery bypass grafting: biological characteristics, functional classification, and clinical choice. Ann Thorac Surg 1999;67:277-84.

25) Angelini GD, Christie MI, Bryan AJ, Lewis MJ. Surgical preparation impairs release of endothelium-derived relaxing factor from human saphenous vein. Ann Thorac Surg 1989;48:417-20.

26) Soyombo AA, Angelini GD, Newby AC. Neointima formation is promoted by surgical preparation and inhibited by cyclic nucleotides in human saphenous vein organ cultures. J Thorac Cardiovasc Surg 1995;109:2-12.

27) Cross KS, Davies MG, el-Sanadiki MN, Murray JJ, Mikat EM, Hagen PO. Long-term human vein graft contractility and morphology: a functional and histopathological study of retrieved coronary vein grafts. Br J Surg 1994;81:699-705.

28) Bonde P, Graham AN, MacGowan SW. Endoscopic vein harvest: advantages and limitations. Ann Thorac Surg 2004;77:2076-82.

29) Kaplan S, Morgan JA, Bisleri G, Cheema FH, Akman HO, Topkara VK, et al. Effects of resveratrol in storage solution on adhesion molecule expression and nitric oxide synthesis in vein grafts. Ann Thorac Surg 2005;80:1773-8.

30) Crook MF, Newby AC, Southgate KM. Expression of intercellular adhesion molecules in human saphenous veins: effects of inflammatory cytokines and neointima formation in culture. Atherosclerosis 2000;150:33-41.

31) Crouch JD, O’Hair DP, Keuler JP, Barragry TP, Werner PH, Kleinman LH. Open versus endoscopic saphenous vein harvesting: wound complications and vein quality. Ann Thorac Surg 1999;68:1513-6.

Keywords : Koroner arter baypas; endoskopik greft çıkarılması; endotelyum; nitrik oksit; venöz greft
Viewed : 10194
Downloaded : 1969