ISSN : 1301-5680
e-ISSN : 2149-8156
Turkish Journal of Thoracic and Cardiovascular Surgery     
The effect of beta-glucan on kidney injury in experimental aortic ischemia-reperfusion
Şenol Gülmen1, Duygu Kumbul Doğuç2, Berit Gökçe Ceylan3, Nesibe Kahraman Çetin4, İbrahim Meteoğlu4, Hüseyin Okutan1, Ahmet Öcal5
1Süleyman Demirel Üniversitesi Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Isparta
2Süleyman Demirel Üniversitesi Tıp Fakültesi, Biyokimya Anabilim Dalı, Isparta
3Süleyman Demirel Üniversitesi Tıp Fakültesi, Anestezi ve Reanimasyon Anabilim Dalı, Isparta
4Adnan Menderes Üniversitesi Tıp Fakültesi, Patoloji Anabilim Dalı, Aydın
5Özel Konak Hastanesi, Kalp ve Damar Cerrahisi Bölümü, Kocaeli
DOI : 10.5606/tgkdc.dergisi.2011.019
Background: In this experimental study, we investigated the effect of Β-glucan on ischemia-reperfusion injury (IR) in kidneys occuring after occlusion-reperfusion of rat infrarenal abdominal aorta (IAA).

Methods: Thirty-two Wistar-albino rats were randomized into four groups with equal number of rats (n=8) as follows: The control group (sham laparotomy), control + Β-glucan, aortic IR, aortic IR + Β-glucan. In the two groups in which aortic IR was used, 120 minutes of ischemia by clamping of the IAA followed by 120 minutes of reperfusion after removal of the clamp were performed. Β-glucan was orally administered at a dose of 50 mg/kg by intragastric gavage twice a day for 10 days. The rats are sacrificed under deep anesthesia and the kidney tissues were removed. The tissue levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase and myeloperoxidase (MPO) were measured and kidney tissue specimens were examined histopatologically.

Results: Biochemical analysis showed that aortic IR significantly increased (p<0.05 vs control) while Β-glucan significantly decreased (p<0.05 vs aortic IR) the kidney tissue levels of MDA, SOD, catalase and MPO. Histologically, in the aortic IR group, focal glomerular necrosis, dilatation of Bowman's capsule, tubular epithelial degeneration, tubular epithelial necrosis, tubular dilatation, interstitial inflammatory infiltration and congestion were significantly increased when compared to the control group (p<0.05 vs control). However, in aortic IR + Β-glucan group, all of these parameters were significantly decreased when compared to the aortic IR group (p<0.05 vs aortic IR).

Conclusion: The results of this experimental study shows that Β-glucan attenuates the kidney injury induced by infrarenal abdominal IR in rats. We think that Β-glucan may decrease oxidative stres, lipid peroxidation, and leucocyte infiltration.

Keywords : Abdominal aorta, beta-glucan; ischemia-reperfusion; kidney injury
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